PAK1 deficiency inhibits CRC progression (IMAGE)
Caption
(A) Relative mRNA expression levels of PAK1 in CRC cell lines (CACO2, DLD1, HT29, LoVo, SW480, and HCT116) compared with normal intestinal epithelial cells (HCoEpiC and NCM460).
(B) Western blotting analysis of PAK1 protein levels in CRC cell lines and normal intestinal epithelial cells (HCoEpiC and NCM460).
(C) Immunohistochemical staining of PAK1 in CRC tissues from the Human Protein Atlas (HPA) database.
(D) Proliferation assays of DLD1 and HT29 cells with PAK1 knockout (KO) compared with wild-type (WT) cells. Cell viability was measured using the CCK8 assay at different time points (0, 24, 48, and 72 h).
(E) Colony formation assays of DLD1 and HT29 cells with PAK1 KO compared with WT cells.
(F) Migration and invasion assays of DLD1 and HT29 cells with PAK1 KO compared with WT cells. The data were presented as mean ± standard deviation, with statistical significance indicated (ns, no significance; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).
Credit
Rongtian Pan, Renrui Zou, Ying Sui, Fei Wu, Dongfeng Wang, Yuan Zhang, Shaorong Yu
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