Figure 1: Design, preparation, and therapeutic mechanisms of PPCi NPs (IMAGE)
Caption
Figure 1: Design, preparation, and therapeutic mechanisms of PPCi NPs. (A) Self-assembly process of PPCi NPs. The opposite electrical properties of PEG- PEI (positively charged) and siRNA (negatively charged) facilitate their electro- static interaction, leading to the formation of PPCi nanoparticles. (B) Schematic illustration of delivery and mechanism of multifunctional drug system. siRNA delivery into the cytosol for the knockdown of the PLK1 gene. The highly cationic nature of the PPCi NPs enhances their endosomal escape feature. The cell’s inward environment provides physicochemical condition changes, which promote the dismantling of the PPCi NPs and the release of the siRNA into the cell’s cytosol. The siRNA achieves PLK1 gene silencing; meanwhile, the p16MIS is carried into the cell’s nucleus and inhibits the binding between CDK-4/6 and Cyclin D, consequently leading to the disruption of the progression of the mi- tosis from the G1 phase to the S phase. The downregulation of PLK1, coupled with the inhibition of the interphase of the mitosis, promotes the apoptosis of tumor cells.
Credit
Milon Essola J, Yang H et al.
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