Figure | DART enables label-free molecular imaging with quantitative virtual staining. (IMAGE)
Caption
Figure | DART enables label-free molecular imaging with quantitative virtual staining. (a) Mesoscale molecular imaging demonstration showing the DART system (inset) acquiring data across a centimeter scale field of view. The topographic height map reveals sample variations from 590 to 635 μm. The recovered nucleic acid mass distribution (0-50.53 femtograms) and protein mass distribution (0-796.90 femtograms) are quantitatively mapped at each pixel without any external labels or stains. (b) Virtual staining based on the quantitative molecular maps produces familiar histological appearances, with nucleic acids (blue) corresponding to cell nuclei and proteins (purple) representing cytoplasm. This physics-based approach provides explainable and reproducible results directly derived from measured molecular content, unlike black-box AI methods.
Credit
Guoan Zheng et al.
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CC BY