Working model explaining how the LoBLH6-LoMYB65 module feedback regulates GA-mediated pollen development. (IMAGE)
Caption
Working model explaining how the LoBLH6-LoMYB65 module feedback regulates GA-mediated pollen development. When the GA level increases, the expression of LoBLH6 and LoMYB65 is induced. BLH6 can independently inhibit the expression of LoGA20ox1, and its interaction with LoMYB65 enhanced the inhibitory effect on LoGA20ox1 expression. Moreover, higher concentrations of GA further promote this interaction, leading to the suppression of GA biosynthesis and maintaining GA contents at a stable level, ensuring normal pollen development occurs. When LoBLH6 was silenced, the LoBLH6-LoMYB65 module in anthers is disrupted. The expression of LoGA20ox1 is no longer repressed by LoBLH6, disrupting the negative feedback regulation of GA synthesis. The elevated GA levels result in premature PCD in the tapetum, abnormal development of anther wall cells, and ultimately the production of aborted pollen. The pointed-end arrows indicate positive effects, the blunt-end arrows indicate negative effects, and the bold line indicate enhanced functions.
Credit
Horticulture Research
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