Figure | Principle of the phasor-FLIM barcoding approach and its application to studying the MNP interactions with small intestinal organoids (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure | Principle of the phasor-FLIM barcoding approach and its application to studying the MNP interactions with small intestinal organoids

Caption

Figure | Principle of the phasor-FLIM barcoding approach and its application to studying the MNP interactions with small intestinal organoids. A, graphical representation of the approach. The polluting polymer is used for producing MNPs, labeled with the near-infrared dye, showing characteristic fluorescence lifetime. MNPs are exposed to the intestinal organoids (basal/apical-out…) with the polarity controlled by the live microscopy. Phasor-FLIM approach is used to analyze the uptake route, mechanism, physiological effect or quantifying the complex mixtures of the absorbed MNPs in the live organoid culture. B, confocal fluorescence images and corresponding phasor plots of organoids incubated with NPs (cyan), co-stained with Nile Red (yellow), demonstrate positive accumulation of only NP B and D, with the characteristic lifetimes confirmed by phasor-FLIM.

Credit

Okkelman, I.A., Zhou, H., Borisov, S.M. et al.

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.