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The procedure of lipid droplet protein co-immunoprecipitation. The experimental process includes ten steps that are indicated in the flowchart. These steps can be summarized into seven key components as illustrated in the figure. They are LD isolation, treatment LD with Triton, centrifugal separation of the lipid layer and protein solution, removal of the lipid layer and collection of LD protein-containing solution, binding of LD proteins to antibody beads, washing of non-specific proteins, and elution of the bait-prey complex. The co-IP samples obtained from the experiment are used for SDS-PAGE analysis and MS-based proteomics
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Xiaochuan Fu, Shuyan Zhang and Pingsheng Liu
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