Tissue-specific expression of SlHAM4. (IMAGE)
Caption
Tissue-specific expression of SlHAM4. (A–O) Histochemical staining for GUS activity in M82 (wild type) and transgenic tomato plants expressing GUS driven by SlHAM4 native promoter (SlHAM4pro::GUS). GUS activity was visualized using the chromogenic substrate X-Gluc after ethanol clearing. (A) Mature embryos extracted from seeds 1 DAI. (B–E) Representative whole seedlings at indicated DAG. Insets in B–D show isolated cotyledons (adaxial side). (F) Adaxial side of the first leaf distal half. (G) Seedling stem. Scale bars = 200 μm (A and F), 500 μm (B), 1 mm (C), 2 mm (D, E, and G). Inset scale bars = 500 μm (B and C), 1 mm (D). cot, cotyledon; hyp, hypocotyl. (J–O) Manual longitudinal (top panel) and cross (bottom panel) sections of anthesis ovary (J) and fruits at indicated developmental stage (K–N). DPA, days post-anthesis. Arrows indicate GUS staining in representative vasculature. Scale bars 1 mm (J–L), 1 cm (M–O). (H, I, O, and P) Histological cross-sections of the SlHAM4pro::GUS-8 tissues stained with Ruthenium red. (H) Seedling stem shown in (G). (O) Pericarp vasculature of mature green fruit shown in (M). Scale bar = 100 μm. (I and P) Higher magnification of the histological sections outlined in (H) and (O) by a black box. Scale bar = 20 μm. p, parenchyma; ph, phloem; pi, internal phloem; pe, external phloem; xy, xylem; se, sieve element cell; cc, companion cell.
Credit
Horticulture Research
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