Mixed transmission sites exist on single spines (IMAGE)
Caption
A) Two-photon image of a basal dendrite of L2/3 neuron loaded with Alexa 594. In this example, the spine is ~40 μm below the surface of the slice. Scale bar, 2 μm. (B) Voltage-clamp traces corresponding to single uncaging trials recorded sequentially at the locations indicated by the orange spots in (A). A 0.5-ms uncaging pulse was delivered at the time indicated by the arrowhead. Laser intensity was the same for each trial. Scale bar, 50 pA and 20 ms. (C) Sample image of L2/3 pyramidal neuron (inset) and enlarged dendritic segment (yellow box). Representative sample traces (color coded to match the spine with uEPSC) of some spines are depicted with uEPSC before TCM (black) and after TCM application (gray). Scale bar, 10 pA and 20 ms; 5 and 50 μm (inset). (D) Summary graph, (E) cumulative probability, and (F) probability density plots of uEPSC amplitudes induced by two-photon uncaging of MNI-glutamate onto a single spine head under the baseline condition (black) and after TCM application (gray). (D) Paired t test; 73/4; T72 = 4.98, ****P < 0.0001. Lines in (F) indicate the mixed Gaussian fit of the uEPSC amplitude distribution.
Credit
Courtesy of Oliver Schlüter Lab/University of Pittsburg
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