Workflow of the GlycoPro platform for sample preprocessing in multi-glycosylation-omics analysis. (IMAGE)
Caption
Starting with 2 µL of human serum, the entire N/O-glycan analysis workflow can be completed within 3.5 h for 384 samples, including enzymatic digestion (for N-glycans) or dissociation (for O-glycans) for 1 h at 50 °C, desalting for 1.5 h, and lyophilization for 1 h. Similarly, the N/O-glycopeptide workflow can be completed within 4.5 h for 384 samples, involving reduction and alkylation for 1 h, enzymatic digestion for 1 h at 50 °C, desalting for 1.5 h, and lyophilization for 1 h.
Credit
Xuejiao Liu et al.
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CC BY-NC-ND