eEF1A2 is tightly correlated with lymph node metastasis and poor prognosis in patients with GBC. (IMAGE)

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eEF1A2 is tightly correlated with lymph node metastasis and poor prognosis in patients with GBC.

Caption

(A) Volcano plot showing DEGs of six pairs of tumors (T) and the corresponding metastasized lymph node tissues (LN). ∣log2FC∣ > 1, adjusted p value (padj) < 0.05. (B) Relative mRNA expression of 8 differentially expressed genes was further quantified by qRT-PCR in 10 paired gallbladder cancer tissues and adjacent normal tissues. (C-D) Expression of eEF1A2 in GBC tumor tissues and paired normal tissues was examined by western blotting (C) and analyzed with ImageJ (D). (E-F) IHC staining was performed in a GBC microarray (cohort 1) to examine the expression of eEF1A2 in GBC tissues and its prognostic value. (E)Representative images of low and high eEF1A2 expression in Cohort 1. Scale bar: 100 μm. (F) Overall survival curves based on tumor eEF1A2 expression using the Kaplan–Meier method and analyzed by log-rank test. (G) Representative images of IHC staining for high and low eEF1A2 expression in GBC cohort 2. Scale bar: 100 μm. (H) IHC scores of eEF1A2 in GBC tumors and adjacent normal tissues in cohort 2 were analyzed. (I) Representative images of IHC staining for eEF1A2 expression in lymph nodes with no metastasis and those with GBC metastasis. Scale bar: 100 μm. (J) IHC scores of eEF1A2 expression in GBC lymph nodes with and without metastasis in cohort 2 were analyzed. (K) The overall survival curves based on tumor eEF1A2 expression in Cohort 2 were drawn using the Kaplan–Meier method and analyzed using the log-rank test. (L-M) Correlation analysis of tumor eEF1A2 expression and lymph node metastasis in gallbladder cancer cohort 1 (L) and cohort 2 (M). Statistical significance between groups was assessed using the chi-square test. Statistical significance between groups was assessed using Student’s t-test. ns, not significant; *, p < 0.05; ** represents p < 0.01; *** represents p < 0.001.

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Genes & Diseases

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