The principle of SIFA and LipoFRET (IMAGE)

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The principle of SIFA and LipoFRET

Caption

A The fluorescence of a fluorophore changes rapidly with its distance to a graphene oxide layer. B The experimental setup of SIFA on TIRF microscopy. C The morphology (upper panel) and the profile of the thickness (lower panel) of a lipid bilayer composed of DOPC/DOPA. D The dependence of the relative intensity on distance in SIFA with different critical distances d0 (Li et al. 2016). E The scheme of LipoFRET. F FRET from one donor to multiple acceptors. G The absorption spectra of TB (blue) and the emission spectra of the donor Alexa Fluor 555-MAL (green). H The dependence of the relative intensity on the distance to the inner surface of the liposome membrane in LipoFRET (Ma et al. 2021)

Credit

Chenguang Yang, Dongfei Ma, Shuxin Hu, Ming Li, Ying Lu

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