Z526's effects on C2C12 myotube atrophy induced by simulated cachectic injuries in vitro. (IMAGE)
Caption
C2C12 myotubes were treated with CMs of cachectic tumor cells or pro-cachectic inflammatory cytokines in the presence or absence of Z526 at various concentrations for 48 h. (A) The structural formula of Z526. (B) Z526's effects on cell viability of C2C12 myotubes. (C–I) Representative images and quantitative analysis results of diameters of C2C12 myotubes induced by (C) CM of C26 tumor cells, (D) CM of LLC tumor cells, (E) CM of HT-1080 tumor cells, (F) CM of PANC-1 tumor cells, (G) CM of MIA PaCa-2 tumor cells, (H) recombinant TNF-α, or (I) recombinant IL-6, respectively. Scale bar, 20 or 50 μm. The data represent mean ± standard error of the mean; ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05. CM, conditioned medium; IL-6, interleukin 6; TNF-α, tumor necrosis factor α.
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