Workflow of the optimized deep imaging protocol for optically cleared skeletal tissue. (IMAGE)

Maximum Academic Press

Workflow of the optimized deep imaging protocol for optically cleared skeletal tissue.

Caption

Workflow of the optimized deep imaging protocol for optically cleared skeletal tissue. Steps for preparing the deep-imaging bone hemisections, including tissue fixation, decalcification, dehydration, embedding, and cryosectioning to generate bone hemisections; and immunostaining, tissue clearing, and mounting, with subsequent imaging. BABB, benzyl alcohol/benzyl benzoate (1:2, v/v) clearing solution.

Credit

Bone Research

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Credit must be given to the creator.

License

CC BY

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