Induction of fibrogenesis-associated genes in human lung fibroblasts on day 3 after N4ICD transduction (IMAGE)

Xia & He Publishing Inc.

Induction of fibrogenesis-associated genes in human lung fibroblasts on day 3 after N4ICD transduction

Caption

contr – untransduced fibroblasts; pCIG – control vector transduced fibroblasts; N4ICD – N4ICD-transduced fibroblasts. (a) Expression efficiency of N4ICD transduction was evaluated by RT-PCR analysis with primers for N4ICD. *** p < 0.001. (b) Morphological changes in N4ICD-transduced cells compared to control ones. (c) RT-PCR results for SNAI1, SNAI2, ACTA2, and COL1A1 in control, pCIG- and N4ICD-transduced cells. *** p < 0.001; ** p < 0.01. (d) and (e). Immunofluorescence labeling analysis of control, pCIG- and N4ICD-transduced cells. Nuclei were stained with DAPI (blue). (d) Immunofluorescence staining with SNAI1 antibodies (green). (e) Immunofluorescence staining with ACTA2 antibodies (red). DAPI, 4′,6-diamidino-2-phenylindole; mRNA, messenger RNA; N4ICD, NOTCH4 intracellular domain; RT-PCR, real-time polymerase chain reaction.

Credit

Nadezhda Bakalenko

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License

CC BY-NC

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