3D Tri-culture Heart-On-A-Chip (IMAGE)
Caption
(a) Design of a microfluidic chip included a top and a bottom channel separated by a central membrane (b) Induced pluripotent stem cell (iPSC)-derived cardiomyocytes and fibroblasts adhered to the top channel, whereas endothelial cells adhered to the bottom channel. (c) Cluster of myocardial cells were seen on the chip. (d) Hematoxylin–eosin staining images of paraffin-embedded sections revealed an approximately 840 μm thick layer of cells (e) Cells oriented in the direction of microfluidic flow. (f) Immunocytochemistry images revealed the structure and location of cTnT, a cardiomyocyte marker.
Credit
Associate Professor Ken Takahashi from Okayama University
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND