Inhibition of miR-10b treats metastatic breast cancer by targeting stem cell-like properties (IMAGE)
Caption
Figure 5: miR-10b inhibition by MN-anti-miR10b decreases Aldefluor accumulation and impairs spheroid formation.
(A) Fluorescence microscopy of representative MDA-MB-231 cells after incubation with Aldefluor reagent. (B) Fluorescence microscopy of representative MCF-7 cells after incubation with Aldefluor reagent. A and B: Mean signal intensity = mean gray value in ImageJ. (C) Mammosphere formation over time of MCF-7 cells treated with MN-anti-miR10b or MN 48 hours prior to (adherent conditions) and during culture in mammosphere medium. (D) Spheroid size (left) and quantity in a field of view (right) of MCF-7 spheroids at Day 7 in treated medium. Plots represent mean ± SEM (left) and mean ± max/min (right). (E) Viability assay of MCF-7 spheroids at Day 7 in treated medium. Plot represents mean ± max/min. A-E: *p < 0.05, **p < 0.01. ****p < 0.0001.
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Halim et al.
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