Adult zebrafish mucus plays a protective role in DSS-induced inflammation (IMAGE)
Caption
(A) Experimental scheme to evaluate the effects of adult zebrafish mucus on larvae induced by DSS. The control group did not receive any treatment.
(B) Representative images of the larvae at 6 Day in control, DSS-induced, and mucus-treated group. ▲ indicates dead larvae.
(C) Cumulative survival rate of three groups (n = 40).
(D) AB staining of the gut from control, DSS, and mucus treatment fish. Scale bar: 50 μm.
(E) The number of goblet cells of gut tissues from three groups (n = 6).
(F) H&E staining of the gut from control, DSS, and mucus treatment fish. EP, epithelium; The black arrow represents the length of the intestinal villi. Scale bar, 20 μm.
(G) The length of gut villus in control, DSS, and mucus groups (n = 6).
(H) Histogram illustrates results from qRT-PCR of transcripts for selected inflammatory factors of DSS-induced, mucus-treated and control zebrafish larvae (n = 6).
Credit
Zixuan Wang
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CC BY-NC-ND